The hospital environment poses a substantial risk for transmission of pathogens. However, assessing cleaning efficiency is difficult and often is carried out by a subjective visual check only. The ATP-bioluminescence assay technology was developed to assist in evaluation of the environmental cleaning process by counting living and non-living organic matter in Relative Light Units (RLUs). In a 1,000-bed university-affiliated hospital the standard practice following patient discharge includes terminal cleaning of the patient unit performed by nursing aides associated with the ward.
Nativ et al. (2013) sought to compare, in RLUs, the outcome of environmental and terminal cleaning in hospital wards using dedicated trained maintenance personnel (strategy 1) versus routine departmental cleaning (strategy 2). An additional purpose was to evaluate the applicability of the ATP-bioluminescence assay technology.
An experimental design with an intervention (strategy 1) and control (strategy 2) was conducted on four comparable medical wards. Two wards used cleaning strategy 1 for terminal cleaning and the other two used cleaning strategy 2 as controls. Ten sites were examined in each ward with ATP-bioluminescence assay: six sites of high risk patient contact areas 30 minutes following terminal cleaning, and 4 sites for general environmental surroundings.
Seven hundred seventy samples were collected; 373 from the intervention group wards and 397 from the control group wards. A statistically significant difference was found in 2 out of 6 high risk patient contact sites in the patient units: 1) the bed rails, with a median of 397 RLUs in the intervention group vs. 752 RLUs in the control group (p<0.001); and 2) the call button, with medians of 338 and 535 RLUs in the intervention and control group, respectively (p<0.002).
The researchers conclude that assigning a dedicated and trained team for terminal cleaning as a cleaning strategy demonstrated superiority at least in 2 out of 6 high-touch surface areas. The ATP-bioluminescence assay technology is a convenient way to check general cleanliness.
Reference: Nativ R, Riven IL and Borer A. Oral presentation O065 at 2nd International Conference on Prevention and Infection Control (ICPIC 2013): Using adenosine tri-phosphate (ATP)-bioluminescence assay to compare outcomes of two strategies to perform environmental cleaning in a hospital setting. Antimicrobial Resistance and Infection Control 2013, 2(Suppl 1):O65 doi:10.1186/2047-2994-2-S1-O65
Disclosures: R. Nativ received grant/research support from M3 Israel and CHEMITEC; I. Livshiz Riven, none declared; A. Borer, none declared.
Vet IP Roundtable 2: Infection Control and Biosecurity Challenges in Veterinary Care
March 31st 2025Veterinary IPs highlight critical gaps in cleaning protocols, training, and biosecurity, stressing the urgent need for standardized, animal-specific infection prevention practices across diverse care settings.
Invisible, Indispensable: The Vital Role of AHRQ in Infection Prevention
March 25th 2025With health care systems under strain and infection preventionists being laid off nationwide, a little-known federal agency stands as a last line of defense against preventable patient harm. Yet the Agency for Healthcare Research and Quality (AHRQ) is now facing devastating cuts—threatening decades of progress in patient safety.
The Sterile Processing Conference Survival Guide: How to Make the Most of Your Next Event
March 25th 2025From expert speakers to cutting-edge tools, sterile processing conferences, like the 2025 HSPA Annual Conference and the SoCal SPA's Spring Conference, offer unmatched opportunities to grow your skills, expand your network, and strengthen your department's infection prevention game.
Redefining Material Compatibility in Sterilization: Insights From AAMI TIR17:2024
March 24th 2025AAMI TIR17:2024 provides updated, evidence-based guidance on material compatibility with sterilization modalities. It offers essential insights for medical device design and ensures safety without compromising functionality.